c29h4 cell signaling technology Search Results


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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
Anti Foxo1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Cell Signaling Technology Inc foxo1 pe
Figure 1. <t>FOXO1</t> limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.
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Image Search Results


Figure 1. FOXO1 limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.

Journal: Cell reports

Article Title: FOXO1 constrains activation and regulates senescence in CD8 T cells.

doi: 10.1016/j.celrep.2020.108674

Figure Lengend Snippet: Figure 1. FOXO1 limits naive CD8+ T cell AP-1 family member expression (A) mRNA abundance (RNA-seq) of WT and Foxo1-KO naive CD8+ T cells. Selected genes are labeled, including members of the AP-1 family. The gray point color indicates no FOXO1 genomic binding detected at day 0; green indicates R1 peaks detected; the size of points indicates the number of FOXO1 genomic binding sites nearest the gene TSS. (B) Intracellular immunostaining determination of indicated protein abundance in naive P14 cells of the indicated genotypes and CD44 expression level. (C) FOXO1 genomic binding (ChIP-seq) and chromatin accessibility (ATAC-seq) for select AP-1 subunits in naive and post-infection (p.i.) (LCMV-ARM) P14 T cells. The y-axis maximum for all ATAC-seq is 75. For (B), data are averaged from 3 experiments with n = 3 mice per group per experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; unpaired Student’s t test was used, and error bars represent means ± SEMs. Informatics experiments are from 1 biological sample per condition.

Article Snippet: Phospho-FoxO1 (CST; 1:1000; Ser256; cat#9461; note antibody is named for human phospho-site) and FoxO1 (CST; 1:1000; C29H4; cat #2880). beta-tubulin (cat# 05-661; 1:1000; Millipore).

Techniques: Expressing, RNA Sequencing, Labeling, Binding Assay, Immunostaining, Quantitative Proteomics, ChIP-sequencing, Infection